RapamycinFungusV1, Main, Exploration, bibRecord, 000858

Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.

Identifieur interne : 000858 ( Main/Exploration ); précédent : 000857; suivant : 000859

Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.

Auteurs : Zhenkai Dai [République populaire de Chine] ; Jianfei Huang [République populaire de Chine] ; Xiaoya Lei [République populaire de Chine] ; Yiming Yan [République populaire de Chine] ; Piaopiao Lu [République populaire de Chine] ; Huanmin Zhang [États-Unis] ; Wencheng Lin [République populaire de Chine] ; Weiguo Chen [République populaire de Chine] ; Jingyun Ma [République populaire de Chine] ; Qingmei Xie [République populaire de Chine]

Source :

Vaccine [ 1873-2518 ] ; 2017.

RBID : pubmed:28049588

Descripteurs français

KwdFr :
- Animaux (MeSH), Anticorps antiviraux (biosynthèse), Autophagie (effets des médicaments et des substances chimiques), Autophagie (génétique), Autophagie (immunologie), Immunité acquise (effets des médicaments et des substances chimiques), Immunité innée (effets des médicaments et des substances chimiques), Interféron gamma (génétique), Interféron gamma (immunologie), Interleukine-10 (génétique), Interleukine-10 (immunologie), Interleukine-2 (génétique), Interleukine-2 (immunologie), Leucose aviaire (génétique), Leucose aviaire (immunologie), Leucose aviaire (prévention et contrôle), Leucose aviaire (virologie), Lymphocytes T (effets des médicaments et des substances chimiques), Lymphocytes T (immunologie), Lymphocytes T (virologie), Maladies de la volaille (génétique), Maladies de la volaille (immunologie), Maladies de la volaille (prévention et contrôle), Maladies de la volaille (virologie), Poulets (MeSH), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Sirolimus (pharmacologie), Vaccination (MeSH), Vaccins antiviraux (administration et posologie), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vaccins à ADN (administration et posologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Vecteurs génétiques (composition chimique), Vecteurs génétiques (immunologie), Virus de la leucose aviaire (effets des médicaments et des substances chimiques), Virus de la leucose aviaire (immunologie), Électroporation (MeSH).
MESH :
- administration et posologie : Vaccins antiviraux, Vaccins à ADN.
- biosynthèse : Anticorps antiviraux.
- composition chimique : Vecteurs génétiques.
- effets des médicaments et des substances chimiques : Autophagie, Immunité acquise, Immunité innée, Lymphocytes T, Virus de la leucose aviaire.
- génétique : Autophagie, Interféron gamma, Interleukine-10, Interleukine-2, Leucose aviaire, Maladies de la volaille, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins à ADN.
- immunologie : Autophagie, Interféron gamma, Interleukine-10, Interleukine-2, Leucose aviaire, Lymphocytes T, Maladies de la volaille, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins à ADN, Vecteurs génétiques, Virus de la leucose aviaire.
- pharmacologie : Sirolimus.
- prévention et contrôle : Leucose aviaire, Maladies de la volaille.
- virologie : Leucose aviaire, Lymphocytes T, Maladies de la volaille.
- Animaux, Poulets, Vaccination, Électroporation.

English descriptors

KwdEn :
- Adaptive Immunity (drug effects), Animals (MeSH), Antibodies, Viral (biosynthesis), Autophagy (drug effects), Autophagy (genetics), Autophagy (immunology), Avian Leukosis (genetics), Avian Leukosis (immunology), Avian Leukosis (prevention & control), Avian Leukosis (virology), Avian Leukosis Virus (drug effects), Avian Leukosis Virus (immunology), Chickens (MeSH), Electroporation (MeSH), Genetic Vectors (chemistry), Genetic Vectors (immunology), Immunity, Innate (drug effects), Interferon-gamma (genetics), Interferon-gamma (immunology), Interleukin-10 (genetics), Interleukin-10 (immunology), Interleukin-2 (genetics), Interleukin-2 (immunology), Poultry Diseases (genetics), Poultry Diseases (immunology), Poultry Diseases (prevention & control), Poultry Diseases (virology), Sirolimus (pharmacology), T-Lymphocytes (drug effects), T-Lymphocytes (immunology), T-Lymphocytes (virology), Vaccination (MeSH), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines (administration & dosage), Viral Vaccines (genetics), Viral Vaccines (immunology).
MESH :
- chemical , administration & dosage : Vaccines, DNA, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Viral.
- chemistry : Genetic Vectors.
- drug effects : Adaptive Immunity, Autophagy, Avian Leukosis Virus, Immunity, Innate, T-Lymphocytes.
- genetics : Autophagy, Avian Leukosis, Interferon-gamma, Interleukin-10, Interleukin-2, Poultry Diseases, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- immunology : Autophagy, Avian Leukosis, Avian Leukosis Virus, Genetic Vectors, Interferon-gamma, Interleukin-10, Interleukin-2, Poultry Diseases, T-Lymphocytes, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- chemical , pharmacology : Sirolimus.
- prevention & control : Avian Leukosis, Poultry Diseases.
- virology : Avian Leukosis, Poultry Diseases, T-Lymphocytes.
- Animals, Chickens, Electroporation, Vaccination.

Abstract

Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.

DOI: 10.1016/j.vaccine.2016.12.034
PubMed: 28049588

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000905
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Le document en format XML

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<author><name sortKey="Zhang, Huanmin" sort="Zhang, Huanmin" uniqKey="Zhang H" first="Huanmin" last="Zhang">Huanmin Zhang</name>
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<author><name sortKey="Lin, Wencheng" sort="Lin, Wencheng" uniqKey="Lin W" first="Wencheng" last="Lin">Wencheng Lin</name>
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<author><name sortKey="Chen, Weiguo" sort="Chen, Weiguo" uniqKey="Chen W" first="Weiguo" last="Chen">Weiguo Chen</name>
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<author><name sortKey="Xie, Qingmei" sort="Xie, Qingmei" uniqKey="Xie Q" first="Qingmei" last="Xie">Qingmei Xie</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.</title>
<author><name sortKey="Dai, Zhenkai" sort="Dai, Zhenkai" uniqKey="Dai Z" first="Zhenkai" last="Dai">Zhenkai Dai</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
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<author><name sortKey="Huang, Jianfei" sort="Huang, Jianfei" uniqKey="Huang J" first="Jianfei" last="Huang">Jianfei Huang</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
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<author><name sortKey="Lei, Xiaoya" sort="Lei, Xiaoya" uniqKey="Lei X" first="Xiaoya" last="Lei">Xiaoya Lei</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yan, Yiming" sort="Yan, Yiming" uniqKey="Yan Y" first="Yiming" last="Yan">Yiming Yan</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lu, Piaopiao" sort="Lu, Piaopiao" uniqKey="Lu P" first="Piaopiao" last="Lu">Piaopiao Lu</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zhang, Huanmin" sort="Zhang, Huanmin" uniqKey="Zhang H" first="Huanmin" last="Zhang">Huanmin Zhang</name>
<affiliation wicri:level="2"><nlm:affiliation>USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823</wicri:regionArea>
<placeName><region type="state">Michigan</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lin, Wencheng" sort="Lin, Wencheng" uniqKey="Lin W" first="Wencheng" last="Lin">Wencheng Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Chen, Weiguo" sort="Chen, Weiguo" uniqKey="Chen W" first="Weiguo" last="Chen">Weiguo Chen</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ma, Jingyun" sort="Ma, Jingyun" uniqKey="Ma J" first="Jingyun" last="Ma">Jingyun Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642</wicri:regionArea>
<wicri:noRegion>Guangdong 510642</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xie, Qingmei" sort="Xie, Qingmei" uniqKey="Xie Q" first="Qingmei" last="Xie">Qingmei Xie</name>
<affiliation wicri:level="1"><nlm:affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China. Electronic address: qmx@scau.edu.cn.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Vaccine</title>
<idno type="eISSN">1873-2518</idno>
<imprint><date when="2017" type="published">2017</date>
</imprint>
</series>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adaptive Immunity (drug effects)</term>
<term>Animals (MeSH)</term>
<term>Antibodies, Viral (biosynthesis)</term>
<term>Autophagy (drug effects)</term>
<term>Autophagy (genetics)</term>
<term>Autophagy (immunology)</term>
<term>Avian Leukosis (genetics)</term>
<term>Avian Leukosis (immunology)</term>
<term>Avian Leukosis (prevention & control)</term>
<term>Avian Leukosis (virology)</term>
<term>Avian Leukosis Virus (drug effects)</term>
<term>Avian Leukosis Virus (immunology)</term>
<term>Chickens (MeSH)</term>
<term>Electroporation (MeSH)</term>
<term>Genetic Vectors (chemistry)</term>
<term>Genetic Vectors (immunology)</term>
<term>Immunity, Innate (drug effects)</term>
<term>Interferon-gamma (genetics)</term>
<term>Interferon-gamma (immunology)</term>
<term>Interleukin-10 (genetics)</term>
<term>Interleukin-10 (immunology)</term>
<term>Interleukin-2 (genetics)</term>
<term>Interleukin-2 (immunology)</term>
<term>Poultry Diseases (genetics)</term>
<term>Poultry Diseases (immunology)</term>
<term>Poultry Diseases (prevention & control)</term>
<term>Poultry Diseases (virology)</term>
<term>Sirolimus (pharmacology)</term>
<term>T-Lymphocytes (drug effects)</term>
<term>T-Lymphocytes (immunology)</term>
<term>T-Lymphocytes (virology)</term>
<term>Vaccination (MeSH)</term>
<term>Vaccines, DNA (administration & dosage)</term>
<term>Vaccines, DNA (genetics)</term>
<term>Vaccines, DNA (immunology)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Vaccines (administration & dosage)</term>
<term>Viral Vaccines (genetics)</term>
<term>Viral Vaccines (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term>
<term>Anticorps antiviraux (biosynthèse)</term>
<term>Autophagie (effets des médicaments et des substances chimiques)</term>
<term>Autophagie (génétique)</term>
<term>Autophagie (immunologie)</term>
<term>Immunité acquise (effets des médicaments et des substances chimiques)</term>
<term>Immunité innée (effets des médicaments et des substances chimiques)</term>
<term>Interféron gamma (génétique)</term>
<term>Interféron gamma (immunologie)</term>
<term>Interleukine-10 (génétique)</term>
<term>Interleukine-10 (immunologie)</term>
<term>Interleukine-2 (génétique)</term>
<term>Interleukine-2 (immunologie)</term>
<term>Leucose aviaire (génétique)</term>
<term>Leucose aviaire (immunologie)</term>
<term>Leucose aviaire (prévention et contrôle)</term>
<term>Leucose aviaire (virologie)</term>
<term>Lymphocytes T (effets des médicaments et des substances chimiques)</term>
<term>Lymphocytes T (immunologie)</term>
<term>Lymphocytes T (virologie)</term>
<term>Maladies de la volaille (génétique)</term>
<term>Maladies de la volaille (immunologie)</term>
<term>Maladies de la volaille (prévention et contrôle)</term>
<term>Maladies de la volaille (virologie)</term>
<term>Poulets (MeSH)</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Sirolimus (pharmacologie)</term>
<term>Vaccination (MeSH)</term>
<term>Vaccins antiviraux (administration et posologie)</term>
<term>Vaccins antiviraux (génétique)</term>
<term>Vaccins antiviraux (immunologie)</term>
<term>Vaccins à ADN (administration et posologie)</term>
<term>Vaccins à ADN (génétique)</term>
<term>Vaccins à ADN (immunologie)</term>
<term>Vecteurs génétiques (composition chimique)</term>
<term>Vecteurs génétiques (immunologie)</term>
<term>Virus de la leucose aviaire (effets des médicaments et des substances chimiques)</term>
<term>Virus de la leucose aviaire (immunologie)</term>
<term>Électroporation (MeSH)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins antiviraux</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Genetic Vectors</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Vecteurs génétiques</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Adaptive Immunity</term>
<term>Autophagy</term>
<term>Avian Leukosis Virus</term>
<term>Immunity, Innate</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Autophagie</term>
<term>Immunité acquise</term>
<term>Immunité innée</term>
<term>Lymphocytes T</term>
<term>Virus de la leucose aviaire</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Autophagy</term>
<term>Avian Leukosis</term>
<term>Interferon-gamma</term>
<term>Interleukin-10</term>
<term>Interleukin-2</term>
<term>Poultry Diseases</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Autophagie</term>
<term>Interféron gamma</term>
<term>Interleukine-10</term>
<term>Interleukine-2</term>
<term>Leucose aviaire</term>
<term>Maladies de la volaille</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antiviraux</term>
<term>Vaccins à ADN</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Autophagie</term>
<term>Interféron gamma</term>
<term>Interleukine-10</term>
<term>Interleukine-2</term>
<term>Leucose aviaire</term>
<term>Lymphocytes T</term>
<term>Maladies de la volaille</term>
<term>Protéines de l'enveloppe virale</term>
<term>Vaccins antiviraux</term>
<term>Vaccins à ADN</term>
<term>Vecteurs génétiques</term>
<term>Virus de la leucose aviaire</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Autophagy</term>
<term>Avian Leukosis</term>
<term>Avian Leukosis Virus</term>
<term>Genetic Vectors</term>
<term>Interferon-gamma</term>
<term>Interleukin-10</term>
<term>Interleukin-2</term>
<term>Poultry Diseases</term>
<term>T-Lymphocytes</term>
<term>Vaccines, DNA</term>
<term>Viral Envelope Proteins</term>
<term>Viral Vaccines</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Sirolimus</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Avian Leukosis</term>
<term>Poultry Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="prévention et contrôle" xml:lang="fr"><term>Leucose aviaire</term>
<term>Maladies de la volaille</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Leucose aviaire</term>
<term>Lymphocytes T</term>
<term>Maladies de la volaille</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Avian Leukosis</term>
<term>Poultry Diseases</term>
<term>T-Lymphocytes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chickens</term>
<term>Electroporation</term>
<term>Vaccination</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Poulets</term>
<term>Vaccination</term>
<term>Électroporation</term>
</keywords>
</textClass>
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<front><div type="abstract" xml:lang="en">Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28049588</PMID>
<DateCompleted><Year>2017</Year>
<Month>12</Month>
<Day>08</Day>
</DateCompleted>
<DateRevised><Year>2017</Year>
<Month>12</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2518</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>35</Volume>
<Issue>5</Issue>
<PubDate><Year>2017</Year>
<Month>02</Month>
<Day>01</Day>
</PubDate>
</JournalIssue>
<Title>Vaccine</Title>
<ISOAbbreviation>Vaccine</ISOAbbreviation>
</Journal>
<ArticleTitle>Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.</ArticleTitle>
<Pagination><MedlinePgn>808-813</MedlinePgn>
</Pagination>
<ELocationID EIdType="pii" ValidYN="Y">S0264-410X(16)31259-2</ELocationID>
<ELocationID EIdType="doi" ValidYN="Y">10.1016/j.vaccine.2016.12.034</ELocationID>
<Abstract><AbstractText>Infection with the avian leukosis virus subgroup J (ALV-J) can lead to neoplastic disease in chickens, inflicting significant economic losses to the poultry industry. Recent reports have identified inhibitory effects of ALV-J on autophagy, a process involving in innate and adaptive immunity. Inspired by this connection between autophagy and immunity, we developed a novel DNA vaccine against ALV-J which includes co-administration of rapamycin to stimulate autophagy. To measure the efficacy of the developed prototype vaccine, five experimental groups of seven-day-old chickens was immunized three times at three-week intervals respectively with vector, pVAX1-gp85, pVAX1-gp85-LC3, pVAX1-gp85+rapamycin and pVAX1-gp85-LC3+rapamycin through electroporation. We then tested their antibody titers, cytokine levels and cellular immune responses. The immunoprotective efficacy of the prototype vaccines against the challenge of the ALV-J GD1109 strain was also examined. The results showed that the combination of pVAX1-gp85-LC3 and rapamycin was able to induce the highest antibody titers, and enhance interleukin(IL)-2, IL-10 and interferon (IFN)-γ expression, and the chickens immunized with the combination of pVAX1-gp85-LC3 and rapamycin showed the highest percentage of CD3+CD8+T lymphocytes. Based on our results, we suggest that stimulating autophagy can improve the efficacy of DNA vaccines and that our DNA vaccine shows the potential of being a candidate vaccine against ALV-J. This study provides a novel strategy for developing vaccines against ALV-J.</AbstractText>
<CopyrightInformation>Copyright © 2016 Elsevier Ltd. All rights reserved.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Dai</LastName>
<ForeName>Zhenkai</ForeName>
<Initials>Z</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Huang</LastName>
<ForeName>Jianfei</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lei</LastName>
<ForeName>Xiaoya</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yan</LastName>
<ForeName>Yiming</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lu</LastName>
<ForeName>Piaopiao</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Huanmin</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>USDA, Agriculture Research Service, Avian Disease and Oncology Laboratory, East Lansing, MI 48823, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lin</LastName>
<ForeName>Wencheng</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Weiguo</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ma</LastName>
<ForeName>Jingyun</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Xie</LastName>
<ForeName>Qingmei</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>College of Animal Science, South China Agricultural University & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, PR China; Key Laboratory of Animal Health Aquaculture and Environmental Control, Guangzhou, Guangdong 510642, PR China; South China Collaborative Innovation Center for Poultry Disease Control and Product Safety, Guangzhou 510642, PR China. Electronic address: qmx@scau.edu.cn.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2016</Year>
<Month>12</Month>
<Day>31</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Netherlands</Country>
<MedlineTA>Vaccine</MedlineTA>
<NlmUniqueID>8406899</NlmUniqueID>
<ISSNLinking>0264-410X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D007376">Interleukin-2</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014765">Viral Vaccines</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>130068-27-8</RegistryNumber>
<NameOfSubstance UI="D016753">Interleukin-10</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>82115-62-6</RegistryNumber>
<NameOfSubstance UI="D007371">Interferon-gamma</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>W36ZG6FT64</RegistryNumber>
<NameOfSubstance UI="D020123">Sirolimus</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D056704" MajorTopicYN="N">Adaptive Immunity</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName>
<QualifierName UI="Q000096" MajorTopicYN="N">biosynthesis</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001343" MajorTopicYN="N">Autophagy</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001353" MajorTopicYN="N">Avian Leukosis</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001354" MajorTopicYN="N">Avian Leukosis Virus</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002645" MajorTopicYN="N">Chickens</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018274" MajorTopicYN="N">Electroporation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005822" MajorTopicYN="N">Genetic Vectors</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007113" MajorTopicYN="N">Immunity, Innate</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016753" MajorTopicYN="N">Interleukin-10</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007376" MajorTopicYN="N">Interleukin-2</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011201" MajorTopicYN="N">Poultry Diseases</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000517" MajorTopicYN="Y">prevention & control</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020123" MajorTopicYN="N">Sirolimus</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014611" MajorTopicYN="Y">Vaccination</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014765" MajorTopicYN="N">Viral Vaccines</DescriptorName>
<QualifierName UI="Q000008" MajorTopicYN="Y">administration & dosage</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Autophagy</Keyword>
<Keyword MajorTopicYN="Y">Avian leucosis virus</Keyword>
<Keyword MajorTopicYN="Y">DNA vaccine</Keyword>
<Keyword MajorTopicYN="Y">Rapamycin</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year>
<Month>07</Month>
<Day>24</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="revised"><Year>2016</Year>
<Month>12</Month>
<Day>12</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2016</Year>
<Month>12</Month>
<Day>14</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2017</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2017</Year>
<Month>12</Month>
<Day>9</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2017</Year>
<Month>1</Month>
<Day>5</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">28049588</ArticleId>
<ArticleId IdType="pii">S0264-410X(16)31259-2</ArticleId>
<ArticleId IdType="doi">10.1016/j.vaccine.2016.12.034</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Guangdong</li>
<li>Michigan</li>
</region>
<settlement><li>Jiangmen</li>
</settlement>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Dai, Zhenkai" sort="Dai, Zhenkai" uniqKey="Dai Z" first="Zhenkai" last="Dai">Zhenkai Dai</name>
</noRegion>
<name sortKey="Chen, Weiguo" sort="Chen, Weiguo" uniqKey="Chen W" first="Weiguo" last="Chen">Weiguo Chen</name>
<name sortKey="Huang, Jianfei" sort="Huang, Jianfei" uniqKey="Huang J" first="Jianfei" last="Huang">Jianfei Huang</name>
<name sortKey="Lei, Xiaoya" sort="Lei, Xiaoya" uniqKey="Lei X" first="Xiaoya" last="Lei">Xiaoya Lei</name>
<name sortKey="Lin, Wencheng" sort="Lin, Wencheng" uniqKey="Lin W" first="Wencheng" last="Lin">Wencheng Lin</name>
<name sortKey="Lu, Piaopiao" sort="Lu, Piaopiao" uniqKey="Lu P" first="Piaopiao" last="Lu">Piaopiao Lu</name>
<name sortKey="Ma, Jingyun" sort="Ma, Jingyun" uniqKey="Ma J" first="Jingyun" last="Ma">Jingyun Ma</name>
<name sortKey="Xie, Qingmei" sort="Xie, Qingmei" uniqKey="Xie Q" first="Qingmei" last="Xie">Qingmei Xie</name>
<name sortKey="Yan, Yiming" sort="Yan, Yiming" uniqKey="Yan Y" first="Yiming" last="Yan">Yiming Yan</name>
</country>
<country name="États-Unis"><region name="Michigan"><name sortKey="Zhang, Huanmin" sort="Zhang, Huanmin" uniqKey="Zhang H" first="Huanmin" last="Zhang">Huanmin Zhang</name>
</region>
</country>
</tree>
</affiliations>
</record>

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	Serveur d'exploration sur la rapamycine et les champignons
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Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.

Efficacy of an autophagy-targeted DNA vaccine against avian leukosis virus subgroup J.

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